Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning (2024)

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Abstract Access options Additional access options: Similar content being viewed by others Overexpression of schizophrenia susceptibility factor human complement C4A promotes excessive synaptic loss and behavioral changes in mice Spatiotemporal expression of IgLON family members in the developing mouse nervous system Elevated expression of complement C4 in the mouse prefrontal cortex causes schizophrenia-associated phenotypes References Author information Authors and Affiliations Rights and permissions About this article Cite this article This article is cited by Cell migration into the damaged brain mediated by increased cell adhesion Neuroimmunomodulatory properties of polysialic acid Interactions between polysialic acid and dopamine-lead compounds as revealed by biochemical and in silico docking simulation analyses Neural glycomics: the sweet side of nervous system functions The cell adhesion protein CAR is a negative regulator of synaptic transmission Comments

Abstract

NEURAL-CELL adhesion molecules (N-CAMs) are members of the immunoglobulin superfamily mediating hom*o- and heterophilic cell-cell interactions. N-CAM exists in various isoforms which are generated by alternative splicing1–3. During embryonic development, N-CAMs are expressed in derivatives of all three germ layers, whereas in the adult animal they are predominantly present in neural tissue. Processes like neurulation4, axonal outgrowth5, histogenesis of the retina6,7 and development of the olfactory system8–10 are correlated with the regulated expression of N-CAMs11–14. We show here that N-CAM-deficient mice generated by gene targeting appear healthy and fertile, but adult mutants show a 10% reduction in overall brain weight and a 36% decline in size of the olfactory bulb. N-CAM deficiency coincides with almost total loss of protein-bound α-(2,8)-linked polysialic acid, a carbohydrate structure thought to be correlated with neural development and plasticity15,16. The animals showed deficits in spatial learning when tested in the Morris water maze17, whereas activity and motor abilities appeared normal.

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Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning (1)

Overexpression of schizophrenia susceptibility factor human complement C4A promotes excessive synaptic loss and behavioral changes in mice

Article 22 December 2020

Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning (2)

Spatiotemporal expression of IgLON family members in the developing mouse nervous system

Article Open access 01 October 2021

Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning (3)

Elevated expression of complement C4 in the mouse prefrontal cortex causes schizophrenia-associated phenotypes

Article 09 April 2021

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Authors and Affiliations

  1. Institute for Genetics, University of Cologne, Zülpicher Strasse 47, D-50674, Cologne, Germany

    Harold Cremer

  2. Institute for Genetics, University of Cologne, D-50931, Cologne, Germany

    Rita Lange,Annette Christoph,Markus Plomann,Gaby Vopper,Jürgen Roes,Dagmar Barthels,Klaus Rajewsky&Wolfgang Wille

  3. Sanders Brown Center on Aging, University of Kentucky, Lexington, Kentucky, 40536-0230, USA

    Russell Brown,Stanley Baldwin,Philipp Kraemer&Stephen Scheff

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Cremer, H., Lange, R., Christoph, A. et al. Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning. Nature 367, 455–459 (1994). https://doi.org/10.1038/367455a0

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Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning (2024)
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